• Product NameFER (Phospho-Tyr402) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of FER only when phosphorylated at tyrosine 402.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 402(V-N-Y(p)-E-E) derived from Human FER .
• Target NameFER
• ModificationPhospho
• Alternative Names    FER; FERT2; TYK3; p94-FER;
• Accession No. Swiss-Prot#: P16591;
  NCBI Gene#: 2241;
  NCBI Protein#: NP_005237.2.
• UniprotP16591
• Gene ID2241;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.

Hao Q.-L., Mol. Cell. Biol. 9:1587-1593(1989).
Lee S.-T., Oncogene 8:3403-3410(1993).
Krolewski J.J., Oncogene 5:277-282(1990).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody #11730 (left)or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from JK cells (Lane 2) and COS7 cells (Lane 3), using FER (Phospho-Tyr402) Antibody #11730. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting