• Product NameILK (Phospho-Ser246) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of ILK only when phosphorylated at serine 246.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK.
• Target NameILK
• ModificationPhospho
• Alternative Names    ILK1; p59ILK; kinase ILK;
• Accession No. Swiss-Prot#: Q13418;
  NCBI Gene#: 3611;
  NCBI Protein#: NP_001014794.1.
• UniprotQ13418
• Gene ID3611;
• SDS-PAGE MW 51kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Hannigan G.E., Nature 379:91-96(1996).
Janji B., Oncogene 19:3069-3077(2000).
Tadic B., Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.

• Immunohistochemical analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody #11733 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting