• Product NameMAP3K1 (Phospho-Thr1400) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1.
• Target NameMAP3K1
• ModificationPhospho
• Alternative Names    M3K1; MAP3K1; MAPKKK1; MEKK1;
• Accession No. Swiss-Prot#: Q13233;
  NCBI Gene#: 4214;
  NCBI Protein#: NP_005912.1.
• UniprotQ13233
• Gene ID4214;
• SDS-PAGE MW 130kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

Schmutz J., Nature 431:268-274(2004).
Xia Y., Genes Dev. 12:3369-3381(1998).
Vinik B.S., Mamm. Genome 6:782-783(1995)

• Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody #11737 (left)or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody #11737. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting