• Product NameMAP3K8 (Phospho-Ser400) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8.
• Target NameMAP3K8
• ModificationPhospho
• Alternative Names    C-COT;; M3K8; MAP3K8; TPL2;
• Accession No. Swiss-Prot#: P41279;
  NCBI Gene#: 1326;
  NCBI Protein#: NP_001231063.1.
• UniprotP41279
• Gene ID1326;
• SDS-PAGE MW 52kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1).

Miyoshi J., Mol. Cell. Biol. 11:4088-4096(1991).
Aoki M., J. Biol. Chem. 268:22723-22732(1993).
Chan A.M.,Oncogene 8:1329-1333(1993).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody #11739 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting