• Product NameMLK1/2 (Phospho-Thr312/266) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 312/266(A-G-T(p)-Y-A) derived from Human MLK1/2.
• Target NameMLK1/2
• ModificationPhospho
• Alternative Names    M3K9; PRKE1; mixed-lineage protein kinase 1;
• Accession No. Swiss-Prot#: P80192/Q02779;
  NCBI Gene#: 4293/4294;
  NCBI Protein#: NP_001271159.1.
• UniprotP80192
• Gene ID4293;
• SDS-PAGE MW 121kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.

Durkin J.T., Biochemistry 43:16348-16355(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Dorow D.S.,Nature 446:153-158(2007).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody #11744 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting