• Product NameMYT1 (Phospho-Ser83) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of MYT1 only when phosphorylated at serine 83.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 83(R-V-S(p)-F-R) derived from Human MYT1.
• Target NameMYT1
• ModificationPhospho
• Alternative Names    PMYT1; PKMYT1; MYT1 kinase;
• Accession No. Swiss-Prot#: Q99640;
  NCBI Gene#: 9088;
  NCBI Protein#: XP_006721039.1.
• UniprotQ99640
• Gene ID9088;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Booher R.N., J. Biol. Chem. 272:22300-22306(1997).
Liu F., Mol. Cell. Biol. 17:571-583(1997).
Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYT1 (Phospho-Ser83) antibody #11745 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting