• Product NameTIE2 (Phospho-Tyr992) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.
• Target NameTIE2
• ModificationPhospho
• Alternative Names    Angiopoietin-1 receptor,EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2;
• Accession No. Swiss-Prot#: Q02763;
  NCBI Gene#: 7010;
  NCBI Protein#: NP_000450.2.
• UniprotQ02763
• Gene ID7010;
• SDS-PAGE MW 126kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Schooley K.A., Baum P.R. Oncogene 8:663-670(1993)
Dunham I. Nature 429:369-374(2004)
Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

• Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody #11756.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting