• Product NameTrk A (Phospho-Tyr757) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of Trk A only when phosphorylated at tyrosine 757.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 757 (E-V-Y(p)-A-I) derived from Human Trk A.
• Target NameTrk A
• ModificationPhospho
• Alternative Names    TRKA; TRK; NTRK1; p140-TrkA;
• Accession No. Swiss-Prot#: P04629;
  NCBI Gene#: 4914;
  NCBI Protein#: NP_002520.2.
• UniprotP04629
• Gene ID4914;
• SDS-PAGE MW 87kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Martin-Zanca D., Mol. Cell. Biol. 9:24-33(1989).
Shelton D.L., J. Neurosci. 15:477-491(1995).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr757) antibody #11757 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting