• Product NameiNOS (Phospho-Tyr151) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 151(Q-Y-Y(p)-G-S) derived from Human iNOS .
• Target NameiNOS
• ModificationPhospho
• Alternative Names    HEP-NOS; NOS2; inducible;
• Accession No. Swiss-Prot#: P35228;
  NCBI Gene#: 4843;
  NCBI Protein#: NP_000616.3.
• UniprotP35228
• Gene ID4843;
• SDS-PAGE MW 131kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).
Sherman P.A., Biochemistry 32:11600-11605(1993).
Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Inos (Phospho-Tyr151) antibody #11766 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting