• Product NameDDR1 (Phospho-Tyr513) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 .
• Target NameDDR1
• ModificationPhospho
• Alternative Names    CAK; RTK6; TRKE; EDDR1;
• Accession No. Swiss-Prot#: Q08345;
  NCBI Gene#: 780;
  NCBI Protein#: NP_001189450.1.
• UniprotQ08345
• Gene ID780;
• SDS-PAGE MW 110kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI).

di Marco E., J. Biol. Chem. 268:24290-24295(1993).
Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993).
Laval S., Cell Growth Differ. 5:1173-1183(1994).

• Western blot analysis of extracts from JK cells (Lane 2), using DDR1 (Phospho-Tyr513) Antibody #11770. The lane on the left is treated with antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody #11770 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting