• Product NamePaxillin (Phospho-Ser178) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe Antibody detects endogenous levels of Paxillin only when phosphorylated at Ser178.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 178(A-L-S(p)-P-L) derived from Human Paxillin.
• Target NamePaxillin
• ModificationPhospho
• Alternative Names    Paxillin; PXN;
• Accession No. Swiss-Prot#: P49023;
  NCBI Gene#: 5829;
  NCBI Protein#: NP_001074324.1.
• UniprotP49023
• Gene ID5829;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

S Chen L.B., Griffin J.D. J. Biol. Chem. 270:5039-5047(1995)
Bell G.I.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases

• Western blot analysis of extracts from HT-29 using Paxillin (Phospho-Ser178) Antibody #11773.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting