• Product NameITCH (Phospho-Tyr420) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH .
• Target NameITCH
• ModificationPhospho
• Alternative Names    AIF4; AIP4; NAPP1;
• Accession No. Swiss-Prot#: Q96J02;
  NCBI Gene#: 83737;
  NCBI Protein#: NP_001244066.1.
• UniprotQ96J02
• Gene ID83737;
• SDS-PAGE MW 102kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain.

Chen X., Genomics 73:238-241(2001).
Deloukas P., Nature 414:865-871(2001).
Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody #11774 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting