• Product NameWWOX (Phospho-Tyr33) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
• Target NameWWOX
• ModificationPhospho
• Alternative Names    FOR; WOX1; Aberrant WW domain-containing oxidoreductase;
• Accession No. Swiss-Prot#: Q96KM3;
  NCBI Gene#: 51741;
  NCBI Protein#: NP_057457.1.
• UniprotQ9NZC7
• Gene ID51741;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.

Bednarek A.K., Cancer Res. 60:2140-2145(2000).
Ried K., Hum. Mol. Genet. 9:1651-1663(2000).
Bednarek A.K., Cancer Res. 61:8068-8073(2001).

• Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody #11779.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody #11779 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting