• Product Namec-Jun(Phospho-Ser73) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.
• Target Namec-Jun
• ModificationPhospho
• Alternative Names    AH119; AP1; Jun A; c-Jun; p39
• Accession No. Swiss-Prot: P05412
  NCBI Protein: NP_002219.1
• UniprotP05412
• Gene ID3725;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

• Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody #11003.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody #11003(left) or the same antibody preincubated with blocking peptide(right).
• Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody #11003.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting