• Product Name4E-BP1 (Phospho-Thr70) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine70 (T-K-T(p)-P-P) derived from Human 4E-BP1.
• Target Name4E-BP1
• ModificationPhospho
• Alternative Names    4EBP1; P/OKCL.6; P/OKCL.6; PHAS-I;
• Accession No. Swiss-Prot#: Q13541;
  NCBI Gene#: 1978;
  NCBI Protein#: NP_004086.1.
• UniprotQ13541
• Gene ID1978;
• SDS-PAGE MW 12kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100  Western Blotting： 1:500~1:1000

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Pause A., Nature 371:762-767(1994).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Haghighat A., EMBO J. 14:5701-5709(1995).

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody #11781 (left)or the same antibody preincubated with blocking peptide (right).
• Western blotting analysis using 4E-BP1 (Phospho-Thr70) Antibody #11781.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting