• Product NameAML1 (Phospho-Ser435) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 435( S-N-S(p)-P-T) derived from Human AML1.
• Target NameAML1
• ModificationPhospho
• Alternative Names    Acute myeloid leukemia 1 protein; CBFA2; CBFA2; alpha 2 subunit; RUNX1
• Accession No. Swiss-Prot#: Q01196;
  NCBI Gene#: 861;
  NCBI Protein#: NP_001001890.1.
• UniprotQ01196
• Gene ID861;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
Nucifora G., Blood 81:2728-2734(1993).

• Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody #11783. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting