• Product NameBRCA1 (Phospho-Ser1457) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1457.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 1457 (L-T-S(p)-Q-K) derived from Human BRCA1.
• Target NameBRCA1
• ModificationPhospho
• Alternative Names    BRCA1; Breast cancer type 1 susceptibility protein; familial breast/ovarian cancer gene 1;
• Accession No. Swiss-Prot#: P38398;
  NCBI Gene#: 672;
  NCBI Protein#: NP_009225.1.
• UniprotP38398
• Gene ID672;
• SDS-PAGE MW 220kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Miki Y., Science 266:66-71(1994).
Smith T.M., Genome Res. 6:1029-1049(1996).
Wilson C.A., Oncogene 14:1-16(1997).

• Western blot analysis of extracts from 293 cells (Lane 2), using BRCA1 (Phospho-Ser1457) Antibody #11787. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting