• Product NameClaudin 5 (Phospho-Tyr217) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Claudin 5 only when phosphorylated at tyrosine 217.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 217 (K-K-N-Y(p)-V) derived from Human Claudin 5.
• Target NameClaudin 5
• ModificationPhospho
• Alternative Names    CLD5; CLDN5; TMDVCF;
• Accession No. Swiss-Prot#: O00501;
  NCBI Gene#: 7122;
  NCBI Protein#: NP_001124333.1.
• UniprotO00501
• Gene ID7122;
• SDS-PAGE MW 23kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome.

Sirotkin H., Genomics 42:245-251(1997).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody #11792 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting