• Product NameClaudin 6 (Phospho-Tyr219) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of Claudin 6 only when phosphorylated at tyrosine 219.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 219 (T-K-N-Y(p)-V) derived from Human Claudin 6.
• Target NameClaudin 6
• ModificationPhospho
• Alternative Names    claudin 6; claudin-6; CLD6; Skullin 2;
• Accession No. Swiss-Prot#: P56747;
  NCBI Gene#: 9074;
  NCBI Protein#: NP_067018.2.
• UniprotP56747
• Gene ID9074;
• SDS-PAGE MW 23kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Claudin 6 Plays a major role in tight junction-specific obliteration of the intercellular space. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO- 3.

Keen T.J., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Troy T., Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
Clark H.F., Genome Res. 13:2265-2270(2003).

• Western blot analysis of extracts from RAW264.7 cells treated with UV using Claudin 6 (Phospho-Tyr219) Antibody #11793.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting