• Product NameClaudin 7 (Phospho-Tyr210) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Claudin 7 only when phosphorylated at tyrosine 210.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 210 (S-K-E-Y(p)-V) derived from Human Claudin 7.
• Target NameClaudin 7
• ModificationPhospho
• Alternative Names    CEPTRL2; claudin 7; CLD7; CPETRL2; CLDN7
• Accession No. Swiss-Prot#: O95471;
  NCBI Gene#: 1366;
  NCBI Protein#: NP_001171951.1.
• UniprotO95471
• Gene ID1366;
• SDS-PAGE MW 32kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells.

Keen T.J.; Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from 3T3 cells (Lane 2), using Claudin 7 (Phospho-Tyr210) Antibody #11794. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting