• Product NameCRMP-2 (Phospho-Thr509) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of CRMP-2 only when phosphorylated at threonine 509.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 509 (S-V-T(p)-P-K) derived from Human CRMP-2.
• Target NameCRMP-2
• ModificationPhospho
• Alternative Names    CRMP2; N2A3; TOAD-64; DPYL2; ULIP2
• Accession No. Swiss-Prot#: Q16555;
  NCBI Gene#: 1808;
  NCBI Protein#: NP_001377.1.
• UniprotQ16555
• Gene ID1808;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

CRMP-2 is an enzyme with dihydropyrimidinase activity. Plays a role in RhoA-dependent signaling, through interaction with and regulation of Rho kinase. Plays a role in neurogenesis. Aberrantly expressed in fetal Down syndrome brain.

Miki Y., Science 266:66-71(1994).
Smith T.M., Genome Res. 6:1029-1049(1996).
Wilson C.A., Oncogene 14:1-16(1997).

• Western blot analysis of extracts from JK cells treated with heat shock using CRMP-2 (Phospho-Thr509) Antibody #11795.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using CRMP-2 (Phospho-Thr509) antibody #11795 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting