• Product NameCyclin C (Phospho-Ser275) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Cyclin C only when phosphorylated at serine 275.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C.
• Target NameCyclin C
• ModificationPhospho
• Alternative Names    CG1C; cyclin C; SRB11;
• Accession No. Swiss-Prot#: P24863;
  NCBI Gene#: 892;
  NCBI Protein#: NP_005181.2.
• UniprotP24863
• Gene ID892;
• SDS-PAGE MW 37kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.

Lew D.J., Cell 66:1197-1206(1991).
Li H., Genomics 32:253-259(1996).
Mungall A.J., Nature 425:805-811(2003).

• Western blot analysis of extracts from 3T3 cells (Lane 2), A549 cells (Lane 3) and Hela cells (Lane 4), using Cyclin C (Phospho-Ser275) Antibody #11797. The lane on the left is treated with antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin C (Phospho-Ser275) antibody #11797 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting