• Product NameEPB41 (Phospho-Tyr660/418) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of EPB41 only when phosphorylated at tyrosine 660/418.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 660/418 (N-I-Y(p)-I-R)derived from Human EPB41.
• Target NameEPB41
• ModificationPhospho
• Alternative Names    E41P; EPB4.1; band 4.1; P4.1;
• Accession No. Swiss-Prot#: P11171;
  NCBI Gene#: 2035;
  NCBI Protein#: NP_001159477.1.
• UniprotP11171
• Gene ID2035;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene, the beta-spectrin gene, or the band 3 gene.

Conboy J.G., Proc. Natl. Acad. Sci. U.S.A. 83:9512-9516(1986).
Tang T.K., Adv. Exp. Med. Biol. 241:81-95(1988).
Tang T.K., Proc. Natl. Acad. Sci. U.S.A. 85:3713-3717(1988).

• Western blot analysis of extracts from HepG2 cells treated with PMA using EPB41 (Phospho-Tyr660/418) Antibody #11799.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting