• Product NameGirdin (Phospho-Ser1417) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of Girdin only when phosphorylated at serine 1417.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 417(Q-K-S(p)-L-T) derived from Human Girdin .
• Target NameGirdin
• ModificationPhospho
• Alternative Names    APE; GIV; GRDN; HkRP1; KIAA1212
• Accession No. Swiss-Prot#: Q3V6T2;
  NCBI Gene#: 55704;
  NCBI Protein#: NP_060554.3.
• UniprotQ3V6T2
• Gene ID55704;
• SDS-PAGE MW 216kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

girdin IS a ubiquitously expressed actin binding protein that plays an important role in the formation of stress fibers and lamellipodia. Subsequent to phosphorylation by Akt, Girdin accumulates at the leading edge of migrating cells and appears to be crucial for the integrity of the actin cytoskeleton and for cell migration.

Enomoto A., Dev. Cell 9:389-402(2005).
Simpson F., Traffic 6:442-458(2005).
Hillier L.W., Nature 434:724-731(2005).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Girdin (Phospho-Ser1417) antibody #11804 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting