• Product Nameb-Catenin(Ab-33) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total b-Catenin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.31~35 (L-D-S-G-I) derived from Human b-Catenin.
• Target Nameb-Catenin
• Alternative Names    CTNNB1; CATNB; CTNB1; CTNNB;
• Accession No. Swiss-Prot: P35222
  NCBI Protein: NP_001091679.1
• UniprotP35222
• Gene ID1499;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 92kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

• Western blot analysis of extracts from Hela and HT29 cells using b-Catenin(Ab-33) Antibody #21211.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Ab-33) Antibody #21211(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using b-Catenin(Ab-33) Antibody #21211.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting