• Product Namep47 phox (Phospho-Ser345) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox.
• Target Namep47 phox
• ModificationPhospho
• Alternative Names    NCF1; P47 phox; NCF-47K;
• Accession No. Swiss-Prot#: P14598;
  NCBI Gene#: 653361;
  NCBI Protein#: NP_000256.4.
• UniprotP14598
• Gene ID653361;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Lomax K.J., Science 245:409-412(1989).
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).

• Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody #11811.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody #11811 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting