• Product NameHS1 (Phospho-Tyr397) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of HS1 only when phosphorylated at tyrosine 397.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 397(G-D-Y(p)-E-E) derived from Human HS1 .
• Target NameHS1
• ModificationPhospho
• Alternative Names    HCLS1; LCKBP1; Hematopoietic cell- specific LYN substrate 1;
• Accession No. Swiss-Prot#: P14317;
  NCBI Gene#: 3059;
  NCBI Protein#: NP_005326.2.
• UniprotP14317
• Gene ID3059;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. Directly associates with HAX-1, through binding to its C-terminal region. May also be involved in the regulation of gene expression.

Kitamura D., Nucleic Acids Res. 17:9367-9379(1989).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from JK cells (Lane 2), using HS1 (Phospho-Tyr397) Antibody #11817.The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting