• Product NamePLD1 (Phospho-Thr147) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.
• Target NamePLD1
• ModificationPhospho
• Alternative Names    PLD 1; hPLD1; Choline phosphatase 1;
• Accession No. Swiss-Prot#: Q13393;
  NCBI Gene#: 5337;
  NCBI Protein#: NP_002653.1.
• UniprotQ13393
• Gene ID5337;
• SDS-PAGE MW 120kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

• Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody #11818.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting