• Product NameYB1 (Phospho-Ser102) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 102(L-R-S(p)-V-G) derived from Human YB1.
• Target NameYB1
• ModificationPhospho
• Alternative Names    CBF-A; NSEP1; EFI-A; MSY-1; YBX1
• Accession No. Swiss-Prot#: P67809;
  NCBI Gene#: 4904;
  NCBI Protein#: NP_004550.2.
• UniprotP67809
• Gene ID4904;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.

Sakura H., Gene 73:499-507(1988).
Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326(1988).
Kolluri R., Nucleic Acids Res. 19:4771-4771(1991).

• Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody #11819.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting