• Product NameHCK (Phospho-Tyr522) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 522.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 522(S-Q-Y(p)-Q-Q) derived from Human HCK .
• Target NameHCK
• ModificationPhospho
• Alternative Names    p59-HCK/p60-HCK; Hemopoietic cell kinas; Tyrosine-protein kinase HCK;
• Accession No. Swiss-Prot#: P08631;
  NCBI Gene#: 3055;
  NCBI Protein#: NP_002101.2.
• UniprotP08631
• Gene ID3055;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Quintrell N., Mol. Cell. Biol. 7:2267-2275(1987) [PubMed: 3496523].
Ziegler S.F.,Mol. Cell. Biol. 7:2276-2285(1987) [PubMed: 3453117].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].

• Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr522) Antibody #11822.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting