• Product NameITGB1 (Phospho-Tyr795) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of ITGB1 only when phosphorylated at tyrosine 795.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 795(P-K-Y(p)-E-G) derived from Human ITGB1 .
• Target NameITGB1
• ModificationPhospho
• Alternative Names    CD29; FNRB; ITB1;
• Accession No. Swiss-Prot#: P05556;
  NCBI Gene#: 3688;
  NCBI Protein#: NP_002202.2.
• UniprotP05556
• Gene ID3688;
• SDS-PAGE MW 140kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Argraves W.S., J. Cell Biol. 105:1183-1190(1987).
Ota T., Nat. Genet. 36:40-45(2004).
Bechtel S., BMC Genomics 8:399-399(2007).

• Western blot analysis of extracts from cos-7 cells (Lane 2), using ITGB1 (Phospho-Tyr795) Antibody #11824. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting