• Product NameSYK (Phospho-Tyr352) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK.
• Target NameSYK
• ModificationPhospho
• Alternative Names    SYK; KSYK; FLJ25043; FLJ37489;
• Accession No. Swiss-Prot#: P43405;
  NCBI Gene#: 6850;
  NCBI Protein#: NP_001167638.1.
• UniprotP43405
• Gene ID6850;
• SDS-PAGE MW 72kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994).
Law C.-L., J. Biol. Chem. 269:12310-12319(1994).
 

• Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody #11827. The lane on the left is treated with antigen-specific peptide.
• Western blot analysis of extracts from Hela cells(1hUV treatment), using Phospho-SYK (Tyr352) Antibody. The lane on the left was treated with blocking peptide.
• Western blot analysis of extracts from Jurkat  H2O2, using Phospho-Syk (Tyr352) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting