• Product NameVAV3 (Phospho-Tyr173) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of VAV3 only when phosphorylated at tyrosine 173.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 173 (E-V-Y(p)-E-D) derived from Human VAV3.
• Target NameVAV3
• ModificationPhospho
• Alternative Names    VAV3; FLJ40431; Guanine nucleotide exchange factor VAV3;
• Accession No. Swiss-Prot#: Q9UKW4;
  NCBI Gene#: 10451;
  NCBI Protein#: NP_006104.4.
• UniprotQ9UKW4
• Gene ID10451;
• SDS-PAGE MW 100kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Trenkle T., Nucleic Acids Res. 26:3883-3891(1998).
Movilla N., Mol. Cell. Biol. 19:7870-7885(1999).

• Western blot analysis of extracts from JK cells using VAV3 (Phospho-Tyr173) Antibody #11830.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting