• Product NameCSFR (Phospho-Tyr708) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe Antibody detects endogenous levels of CSF1R only when phosphorylated at tyrosine 708.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 708 (K-K-Y(p)-V-R) derived from Human CSFR .
• Target NameCSFR
• ModificationPhospho
• Alternative Names    C-FMS; CD115; CSF1R; FIM2; FMS
• Accession No. Swiss-Prot#: P07333;
  NCBI Gene#: 1436;
  NCBI Protein#: NP_001275634.1.
• UniprotP07333
• Gene ID1436;
• SDS-PAGE MW 200kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development.

Hampe A., Oncogene Res. 4:9-17(1989) .
Andre C., Genomics 39:216-226(1997).

• Western blot analysis of extracts from HepG2 cells using CSFR (Phospho-Tyr708) Antibody #11832.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting