• Product NameEPHA2/5 (Phospho-Tyr594) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe Antibody detects endogenous levels of EPHA2 only when phosphorylated at tyrosine 594.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 594 (H-T-Y(p)-E-D) derived from Human EPHA2/5.
• Target NameEPHA2/5
• ModificationPhospho
• Alternative Names    ARCC2; ECK; EPHA2;
• Accession No. Swiss-Prot#: P29317/P54756;
  NCBI Gene#: 1969/2044;
  NCBI Protein#: NP_004422.2.
• UniprotP29317
• Gene ID1969;
• SDS-PAGE MW 110kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from JK cells (Lane 2), using EPHA2/5 (Phospho-Tyr594) Antibody #11833. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting