• Product NameEPHA4 (Phospho-Tyr596) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 596 (R-T-Y(p)-V-D) derived from Human EPHA4 .
• Target NameEPHA4
• ModificationPhospho
• Alternative Names    EK8; hEK8; EPH-like kinase 8;
• Accession No. Swiss-Prot#: P54764;
  NCBI Gene#: 2043;
  NCBI Protein#: NP_004429.1.
• UniprotP54764
• Gene ID2043;
• SDS-PAGE MW 110kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.

Rikova K., Cell 131:1190-1203(2007) [PubMed: 18083107].
Richter M., J. Neurosci. 27:14205-14215(2007) [PubMed: 18094260].
Fu W.Y., Nat. Neurosci. 10:67-76(2007) [PubMed: 17143272].

• Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody #11834. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting