• Product NameANXA2 (Phospho-Ser26) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of ANXA2 only when phosphorylated at serine 26.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 26 (Y-G-S(p)-V-K) derived from Human ANXA2.
• Target NameANXA2
• ModificationPhospho
• Alternative Names    Annexin A2; Annexin II; Annexin-2; CAL1H; Calpactin I heavy chain
• Accession No. Swiss-Prot#: P07355;
  NCBI Gene#: 302;
  NCBI Protein#: NP_001002857.1.
• UniprotP07355
• Gene ID302;
• SDS-PAGE MW 38kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.

Huang K.-S., Cell 46:191-199(1986).
Spano F., Gene 95:243-251(1990).
Bechtel S., BMC Genomics 8:399-399(2007).

• Western blot analysis of extracts from HepG2 cells JK cells and K562 cells using ANXA2 (Phospho-Ser26) Antibody #11839.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting