• Product Name4E-BP1 (Ab-37) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total 4E-BP1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.35~39 (S-T-T-P-G) derived from Human 4E-BP1.
• Target Name4E-BP1
• Alternative Names    EIF4EBP1; PHAS-1;
• Accession No. Swiss-Prot: Q13541
  NCBI Protein: NP_004086.1
• UniprotQ13541
• Gene ID1978;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 18kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. Genes Dev Nov. 2005 Feb 15.
Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64.
Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37.
Lal L, et al.(2005) Blood;105(4): 1669-7.

• Western blot analysis of extracts from HepG2 and 293 cells using 4E-BP1 (Ab-37) Antibody #21215.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-37) Antibody #21215 (left) or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting