• Product NameGFAP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total GFAP protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human GFAP .
• Target NameGFAP
• Alternative Names    GFAP; FLJ45472;
• Accession No. Swiss-Prot:P14136
  NCBI Gene ID:2670
• UniprotP14136
• Gene ID2670;
• SDS-PAGE MW 50KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin-forming Schwann cells in the peripheral nervous system (3).

• Western blot analysis of extracts of various cell lines, using GFAP at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using GFAP at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using GFAP at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of U-251MG cells using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of rat brain using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of mouse brain using GFAP at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting