• Product NameLMNA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total LMNA protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human LMNA.
• Target NameLMNA
• Alternative Names    LMNA; CDCD1; CDDC; CMD1A; CMT2B1
• Accession No. Swiss-Prot:P02545
  NCBI Gene ID:4000
• UniprotP02545
• Gene ID4000;
• SDS-PAGE MW 65KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200
  IP 1:50 - 1:200

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

• Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using Lamin A/C at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37˚C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37˚C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37˚C for 20 hours or treated by Paclitaxel (100 nM) at 37˚C for 20 hours.
• Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using Lamin A/C . Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting