• Product NameMLH1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MLH1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human MLH1 (NP_000240.1).
• Target NameMLH1
• Alternative Names    MLH1;COCA2;FCC2;HNPCC;HNPCC2;hMLH1
• Accession No. Uniprot:P40692
  GeneID:4292
• UniprotP40692
• Gene ID4292
• SDS-PAGE MW 85KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

• Western blot analysis of extracts of Mouse thymus cells, using MLH1 antibody.
• Western blot analysis of extracts of various cell lines, using MLH1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting