• Product NameMet(Ab-1234) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Met protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.1232~1236 (K-E-Y-Y-S) derived from Human Met.
• Target NameMet
• Alternative Names    HGF receptor; HGF-SF receptor; Met proto-oncogene tyrosine kinase; c-met; kinase Met
• Accession No. Swiss-Prot: P08581
  NCBI Protein: NP_000236.2
• UniprotP08581
• Gene ID4233;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 156kd
  Western blotting: 1:500~1:1000
  

Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

Gherardi E. et al. (2003).Proc Natl Acad Sci U S A. 100(21): 12039-12044.
Shiu SH. et al. (2001) Proc Natl Acad Sci U S A. 98(19): 10763-10768.
Hughes AL. et al. (2001) Genome Res. 11(5): 771-780.
Onuchic LF. et al. (2002) Am J Hum Genet. 70(5): 1305-1317.

• Western blot analysis of extracts from HepG2 cells using Met(Ab-1234) Antibody #21220 and the same antibody preincubated with blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting