• Product NamePPARG Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PPARG protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human PPARG .
• Target NamePPARG
• Alternative Names    PPARG; PPARgamma; CIMT1; PPARG2; PPARG1
• Accession No. Swiss-Prot:P37231
  NCBI Gene ID:5468
• UniprotP37231
• Gene ID5468;
• SDS-PAGE MW 58KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).

• Confocal immunofluorescence analysis of HeLa cells using PPAR gamma Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of U-2 OS cells using PPAR gamma Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using PPAR gamma at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using PPAR gamma at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded Rat heart using PPAR at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded Human colon using PPAR at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded Mouse lung using PPAR at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting