• Product NameIRS-1(Ab-636) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total IRS-1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1.
• Target NameIRS-1
• Alternative Names    IRS-1; IRS1;
• Accession No. Swiss-Prot: P35568
  NCBI Protein: NP_005535.1
• UniprotP35568
• Gene ID3667;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 180kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360

• Western blot analysis of extracts from 293 cells using IRS-1(Ab-636) Antibody #21223.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Ab-636) Antibody #21223(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using IRS-1(Ab-636) Antibody #21223.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting