• Product NameS6 Ribosomal Protein(Ab-235) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total S6 Ribosomal protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.
• Target NameS6 Ribosomal Protein
• Alternative Names    NP33; RPS6; RS6
• Accession No. Swiss-Prot: P62753
  NCBI Protein: NP_001001.2
• UniprotP62753
• Gene ID6194;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 32kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.

• Western blot analysis of extracts from HepG2 and 293 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.
• Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using S6 Ribosomal Protein(Ab-235) Antibody #21225(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF7 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting