• Product NameTPMT Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total TPMT protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TPMT.
• Target NameTPMT
• Alternative Names    TPMT; Thiopurinemethyltransferase; thiopurineSmethyltransferase;
• Accession No. Swiss-Prot:P51580
  NCBI Gene ID:7172
• UniprotP51580
• Gene ID7172;
• SDS-PAGE MW 28KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:10 - 1:100

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

• Western blot analysis of extracts of K562 cells, using TPMT at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting