• Product NameMAGED1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MAGED1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MAGED1.
• Target NameMAGED1
• Alternative Names    MAGED1; DLXIN-1; NRAGE;
• Accession No. Swiss-Prot:Q9Y5V3
  NCBI Gene ID:9500
• UniprotQ9Y5V3
• Gene ID9500;
• SDS-PAGE MW 86KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

MAGE-D1, also known as NRAGE or Dlxin-1, is a member of the MAGE family of proteins. Identified as a p75 neurotrophin receptor intracellular binding protein, MAGE-D1 induces developmental apoptosis of motoneurons, and is required for p75NTR-dependent apoptosis in sympathetic neurons. It is suggested that MAGE-D1 can suppress the motility and adhesion response of tumor cells. By forming a hetercomplex with Dlx/Msx family homeodomain proteins and Necdin, MAGE-D1 modulates the function of Dlx/Msx homeodomain during terminal differentiation and maturation of neurons. MAGE-D1 is also involved in the phosphorylation of IKK-alpha/beta, and subsequent transcriptional activation of the p65 subunit of NF-kappaB, via the XIAP-Tak1-Tab1 complex. (20100315, 19639218, 17453828, 15272023) G.

• Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using MAGED1 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting