• Product Namec-Kit(Ab-721) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total c-Kit protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.719~723 (N-E-Y-M-D) derived from Human c-Kit.
• Target Namec-Kit
• Alternative Names    KIT; CD117; SCFR; PBT;
• Accession No. Swiss-Prot: P11362
  NCBI Protein: NP_000213.1
• UniprotP11362
• Gene ID2260;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 145kd
  Western blotting: 1:500~1:1000
  

This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Kimura W,et al.(2007)Hepatogastroenterology.54(80):2203-8.
Naeem M,et al.(2002)Hum Pathol. 33(12):1182-7.
Jankulovski N, et al.(2006)Prilozi. 27(2):59-70.

• Western blot analysis of extracts from A431 cells using c-Kit(Ab-721) Antibody #21232 and the same antibody preincubated with blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting