• Product NameeNOS(Ab-495) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsIF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total eNOS protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.493~497 (K-K-T-F-K) derived from Human eNOS.
• Target NameeNOS
• Alternative Names    Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII
• Accession No. Swiss-Prot: P29474
  NCBI Protein: NP_000594.2
• UniprotP29474
• Gene ID4846;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 140kd
  Immunofluorescence: 1:100~1:200

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Moreno H Jr.(2008)Breast Cancer Res Treat. 109(1):181-2.
Lorenz M,et al.(2007)FASEB J. May;21(7):1556-64.
Lin HI,et al.(2006)Transplant Proc. Sep;38(7):2203-6.

• Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Ab-495) Antibody #21235.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting