• Product NameFEN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total FEN1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human FEN1.
• Target NameFEN1
• Alternative Names    FEN1; FEN-1; MF1; RAD2;
• Accession No. Swiss-Prot:P39748
  NCBI Gene ID:2237
• UniprotP39748
• Gene ID2237;
• SDS-PAGE MW 43KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100
  IP 1:50 - 1:200

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

• Western blot analysis of extracts of various cell lines, using FEN1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse liver using FEN1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of A549 cells using FEN1 .
• Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using FEN1 .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting